Long non-coding RNA produced by RNA polymerase V determines boundaries of heterochromatin

被引:69
|
作者
Bohmdorfer, Gudrun [1 ]
Sethuraman, Shriya [2 ]
Roewley, M. Jordan [1 ]
Krzyszton, Michal [3 ]
Rothi, M. Hafiz [1 ]
Bouzit, Lilia [1 ]
Wierzbicki, Andrzej T. [1 ]
机构
[1] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Bioinformat Grad Program, Ann Arbor, MI 48109 USA
[3] Univ Warsaw, Inst Genet & Biotechnol, Fac Biol, Warsaw, Poland
来源
ELIFE | 2016年 / 5卷
基金
奥地利科学基金会; 美国国家科学基金会;
关键词
DIRECTED DNA METHYLATION; ARABIDOPSIS-THALIANA; POL IV; EPIGENETIC PATHWAY; PROCESSING CENTER; SIRNA BIOGENESIS; CHROMATIN; TRANSCRIPTION; METHYLTRANSFERASES; ARGONAUTE4;
D O I
10.7554/eLife.19092
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
RNA-mediated transcriptional gene silencing is a conserved process where small RNAs target transposons and other sequences for repression by establishing chromatin modifications. A central element of this process are long non-coding RNAs (lncRNA), which in Arabidopsis thaliana are produced by a specialized RNA polymerase known as Pol V. Here we show that non-coding transcription by Pol V is controlled by preexisting chromatin modifications located within the transcribed regions. Most Pol V transcripts are associated with AGO4 but are not sliced by AGO4. Pol V-dependent DNA methylation is established on both strands of DNA and is tightly restricted to Pol V-transcribed regions. This indicates that chromatin modifications are established in close proximity to Pol V. Finally, Pol V transcription is preferentially enriched on edges of silenced transposable elements, where Pol V transcribes into TEs. We propose that Pol V may play an important role in the determination of heterochromatin boundaries.
引用
收藏
页数:24
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