Isolation of total RNA from baker's yeast

被引：2
|
作者：
Yamkovaya, TV
Khomov, VV
Zagrebelny, SN
Yamkovoy, VI
机构：
[1] Novosibirsk State Pedag Univ, Novosibirsk 610126, Russia
[2] Novosibirsk State Univ, Novosibirsk 630090, Russia
[3] Russian Acad Sci, Novosibirsk Inst Organ Chem, Siberian Div, Novosibirsk 630090, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2006年 / 42卷 / 01期
关键词：
Sodium; Precipitation; Sulfate; Biomass; Filtration;
D O I：
10.1134/S0003683806010133
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A simple method of production of total RNA from baker's yeast was developed. Total RNA was isolated from yeast (Saccharomyces cerevisiae) biomass using lysis with sodium dodecyl sulfate at 100 degrees C for 40-60 min and subsequent precipitation of the target product with 3 M NaCl. The preparation obtained was characterized in detail: yield of total RNA from 1 kg of pressed yeast, 9.25 g; optical density at 260 nm of 1 mg of RNA dissolved in 1 ml of water, 20.2 U; content of the acid-soluble fraction, 2.02%; and protein content, 1.8%. Total tRNA was isolated from total RNA by fractional precipitation with ethanol followed by gel filtration.
引用
收藏
页码：84 / 88
页数：5
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