In situ isobaric lipid mapping by MALDI-ion mobility separation-mass spectrometry imaging

被引：36
|
作者：
Fu, Tingting [1 ]
Oetjen, Janina [2 ]
Chapelle, Manuel [2 ]
Verdu, Alexandre [2 ]
Szesny, Matthias [2 ]
Chaumot, Arnaud [3 ]
Degli-Esposti, Davide [3 ]
Geffard, Olivier [3 ]
Clement, Yohann [1 ]
Salvador, Arnaud [1 ]
Ayciriex, Sophie [1 ]
机构：
[1] Univ Claude Bernard Lyon 1, Univ Lyon, Inst Sci Analyt, CNRS,UMR 5280, 5 Rue Doua, F-5280 Villeurbanne, France
[2] Bruker Daltonik GmbH, Bremen, Germany
[3] INRAE, UR RiverLy, Lab Ecotoxicol, Villeurbanne, France
来源：
JOURNAL OF MASS SPECTROMETRY | 2020年 / 55卷 / 09期
关键词：
ion mobility separation; lipid isomers; MALDI; mass spectrometry imaging; TIMS;
D O I：
10.1002/jms.4531
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustaceanGammarus fossarumusing matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high-resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.
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页数：6
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