A thermostable D-hydantoinase isolated from a Mesophilic bacillus sp AR9

被引：16
|
作者：
Sharma, R [1 ]
Vohra, RM [1 ]
机构：
[1] INST MICROBIAL TECHNOL, BIOCHEM ENGN RES & PROC DEV CTR, CHANDIGARH 160036, INDIA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 234卷 / 02期
关键词：
D O I：
10.1006/bbrc.1997.6659
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A thermostable hydantoinase has been characterized from a mesophilic Bacillus sp.AR9. The hydantoinase produced by this Bacillus sp.AR9 is strictly D-specific and is constitutively produced with high yields (4500 U/ml) in this strain. The enzyme is not only alkalo- and thermostable but has a pH and temperature optimum of 9.5 and 65 degrees C, respectively, which is advantageous for the bioconversion of DL-5-monosubstituted-hydantoin derivatives, The enzyme has a half life of 80 minutes at 70 degrees C and loses only 33% of its activity in 4 hr at 60 degrees C, The enzyme has a broad substrate specificity with a maximum of 100% with hydantoin and about 26% with dihydrouracil. Co++ ions enhance the activity of the enzyme by more than 60%. (C) 1997 Academic Press.
引用
收藏
页码：485 / 488
页数：4
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