Spectrofluorometric determination of hydrogen peroxide based on oxidative catalytic reactions of p-hydroxyphenyl derivatives with metal complexes of thiacalix[4]arenetetrasulfonate on a modified anion-exchanger

The peroxidase-like catalytic activity of metal complexes of thiacalix[4]arenetetrasulfonate (TCAS[4]) on a modified anion-exchanger (Men+-TCAS[4](A-500); Men+ = H-2, Fe3+, Fe2+, Mn3+, Co3+, Co2+, Cu2+, Zn2+, Ni2+) for the oxidation of p-hydroxyphenyl derivatives to produce fluorescent substances in the presence of hydrogen peroxide has been investigated. Among the Men+-TCAS[4](A-500) tested, Fe3+-TCAS[4](A-500) exhibited the highest level of catalytic activity for the oxidation of p-acetoamidophenol in a carbonate buffer solution of pH 10. The catalytic activity of Fe3+-TCAS[4]A-500 was then used for the spectrofluorometric determination of hydrogen peroxide. The calibration curve for the Fe3+-TCAS[4](A-500) method was linear over a range spanning from 0.1 to 5.0 mug of hydrogen peroxide in a 1.0 ml sample solution.