Cloning and expression of the rpoH gene from Escherichia coli and its product purification

被引：0
|
作者：
Chen, YH [1 ]
Hua, ZC [1 ]
Dong, C [1 ]
Ma, Z [1 ]
Zhu, DX [1 ]
机构：
[1] NANJING UNIV, DEPT BIOCHEM, NANJING 210093, PEOPLES R CHINA
来源：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 1996年 / 28卷 / 02期
关键词：
sigma(32); heat shock protein; gene expression; nickel-iminodiacetic acid affinity chromatography;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The rpoH gene, encoding the heat shock protein transcription factor sigma(32), was obtained by PCR and cloned into plasmid pUHE with the tac promoter. Upon induction with IPTG, it directed the expression of sigma(32) tailed with six additional histidine residues at its C-terminus. The histidine-tagged sigma(32) was purified with nickel-iminodiacetic acid affinity chromatography. Using S-35 radiolabeling methods in vivo, we found that even at low temperature the expression of sigma(32)-6 His may markedly increase the synthesis of the heat shock proteins such as GroEL, DnaK, and HtpH.
引用
收藏
页码：170 / 176
页数：7
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