The Effect of Overexpression Of α (1,3)- upregulating VII on Adhesive Capability of Human Colon Carcinoma HT-29 Cells to HUVECs

There are sufficient evidences that Lewis antigens are tumor-associated molecules and correlated to high grade and poor prognosis tumors. In this study, we investigated the effect of (alpha 1,3)-fucosyltransferaseVII overexpression on the synthesis of sLex and adhesive capability of human colon carcinoma HT-29 cells to HUVECs.The pIRES2-EGFP-FucTVII eukaryotic expression vector were transiently transfected into HT-29 cells. The changes of FucT VII protein and mRNA expression were determined by flow cytomet- ry and Real-Time PCR; the effect of FucT VII overexpression on synthesis of its downstream product-sLex is detected by Flow cytometry; Rose-Bengal method is used to assay the capability of HT-29 cell adhesion to HUVECs. Results: Eukaryotic expression vector pIRES2-EGFP-FucTVII was successfully transfected into HT-29 cells and made FucTVII overexpressed; compared with that of control group, expression level of the sLeX on the surface of FucTVII transfected HT-29 cells was significantly higher; FucTVII overexpression could enhance the adhesive capability of HT-29 cells to HUVECs. Our data suggest that overexpression of FucTVII could strengthen adhesion of sLeX-mediated HT-29 cells to HUVECs through upregulating sLeX synthesis.