Antioxidant and Antimicrobial Properties of Annona Muricata Leaf and Pulp Methanolic Extract

Context: Annona muricata is a medicinal plant from the Annonaceae family originally comes from the native America but is now become established in many tropical countries, especially in Malaysia. With the advancement of science and technology further study have been done to establish the function of Annona muricata in the healthcare industries. Some phytochemical constituents are acetogenins, coumarins, phenolic, alkaloids and flavonoids. The phytochemical content is the chemical constituent that gives the antioxidants, anti-microbial, anti-diabetics, and many other health benefits.Aims: This study aimed to identify the phytochemicals in Annona muricata leaves and pulp extracts specifically phenolic and flavonoids and, to evaluate and compare the antioxidant and anti-microbial activity between the two parts of the plant.Methods: The pulp and leave extract were obtained from a Negeri Sembilan. Antioxidant tests included FRAP, DPPH, and TPC were conducted, while antimicrobial testing was done against S.aureus using the disc diffusion method. The concentration of the sample used in the study was 25 mu g/ml, 50 mu g/ml, 100 mu g/ml, 200 mu g/ml and 400 mu g/ml.Results: The result of the study shows that both parts of leaf and pulp of Annona muricata extracts possess a good antioxidant level with the concentration of 25 mu g, 50 mu 100 mu g, 200 mu and 400 mu g. But in contrast those concentration does not show any significant antimicrobial activity for both extracts. When comparing between the pulp and leaves extracts, the leaves extract elicits higher content of antioxidant level and higher sensitivity towards microorganism compared to pulp extracts.