Detection of Carbofuran with Immobilized Acetylcholinesterase Based on Carbon Nanotubes-Chitosan Modified Electrode

被引：3

作者：

Zhang, Shuping ^{[1
,2
]}

Li, Shaoyang ^{[2
]}

Ma, Jie ^{[1
]}

Xiong, Fei ^{[1
]}

Qu, Song ^{[1
]}

机构：

[1] Univ Shanghai Sci & Technol, Coll Sci, Shanghai 200093, Peoples R China

[2] Univ Shanghai Sci & Technol, Coll Environm & Architecture, Shanghai 200093, Peoples R China

来源：

JOURNAL OF NANOMATERIALS | 2013年 / 2013卷

关键词：

D O I：

10.1155/2013/751236

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A sensitive and stable enzyme biosensor based on efficient immobilization of acetylcholinesterase (AChE) to MWNTs-modified glassy carbon electrode (GCE) with chitosan (CS) by layer-by-layer (LBL) technique for rapid determination of carbofuran has been devised. According to the inhibitory effect of carbamate pesticide on the enzymatic activity of AChE, we use carbofuran as a model pesticide. The inhibitory effect of carbofuran on the biosensor was proportional to concentration of carbofuran in the range from 10(-10) g/L to 10(-3) g/L with a detection limit of 10(-12) g/L. This biosensor is a promising new method for pesticide analysis.

引用

页数：4

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