PEROXIDASE-LINKED ASSAY FOR DETECTION OF ANTIBODIES AGAINST BOVINE LEUKOSIS VIRUS

被引：0

作者：

de Castro, Clarissa C. ^{[1
]}

Nunes, Cristina F. ^{[1
]}

Finger, Paula F. ^{[1
]}

Siedler, Bianca S. ^{[1
]}

Dummer, Luana ^{[2
]}

de Lima, Marcelo ^{[1
]}

Leite, Fabio P. L. ^{[2
]}

Fischer, Geferson ^{[1
]}

Vargas, Gilberto D'A. ^{[1
]}

Huebner, Silvia de O. ^{[1
]}

机构：

[1] Univ Fed Pelotas, Lab Virol & Immunol, BR-96010900 Pelotas, RS, Brazil

[2] Univ Fed Pelotas, Technol Dev Ctr, Ctr Biotechnol, Bacteriol Lab, BR-96010900 Pelotas, RS, Brazil

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2013年 / 34卷 / 04期

关键词：

antibodies; bovine leukosis virus; diagnostic; immunoperoxidase; serology; test; POLYMERASE-CHAIN-REACTION; LEUKEMIA-VIRUS; MYCOPLASMA CONTAMINATION; PROVIRAL DNA; INFECTION; BLOOD; PCR;

D O I：

10.1080/15321819.2012.747442

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27 samples found to be positive by PLA. PLA showed 100% of sensitivity when compared with AGID and ELISA. Specificity was 64.8% and 78%, respectively (kappa coefficients were 0.70 and 0.83). These findings indicate that PLA can be used as an alternative method for the diagnosis of BLV infection in cattle.

引用

页码：376 / 383

页数：8

共 50 条

[1] A DIRECT NEUTRALIZING PEROXIDASE-LINKED ANTIBODY-ASSAY FOR DETECTION AND TITRATION OF ANTIBODIES TO BOVINE VIRAL DIARRHEA VIRUS
HYERA, JMK
LIESS, B
FREY, HR
[J]. JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1987, 34 (03): : 227 - 239
[2] THE NEUTRALIZING PEROXIDASE-LINKED ASSAY FOR DETECTION OF ANTIBODY AGAINST SWINE FEVER VIRUS
TERPSTRA, C
BLOEMRAAD, M
GIELKENS, ALJ
[J]. VETERINARY MICROBIOLOGY, 1984, 9 (02) : 113 - 120
[3] STUDIES ON BOVINE LEUKOSIS .6. ENZYME LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF ANTIBODIES TO BOVINE LEUKOSIS VIRUS
RESSANG, AA
GIELKENS, ALJ
QUAK, S
MASTENBROEK, N
TUPPERT, C
DECASTRO, A
[J]. ANNALES DE RECHERCHES VETERINAIRES, 1978, 9 (04): : 663 - 666
[4] TEST PROTOCOL OF AN ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) FOR THE DETECTION OF ANTIBODIES AGAINST BOVINE LEUKOSIS VIRUS
GIELKENS, ALJ
RESSANG, AA
IJZERMANN, J
QUAK, J
[J]. VETERINARY QUARTERLY, 1981, 3 (01) : 34 - 37
[5] A Peroxidase-linked Spectrophotometric Assay for the Detection of Monoamine Oxidase Inhibitors
Zhi, Kangkang
Yang, Zhongduo
Sheng, Jie
Shu, Zongmei
Shi, Yin
[J]. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH, 2016, 15 (01): : 131 - 139
[6] Detection of antibodies of bovine leukosis with immunoperoxidase assay (PLA)
Buzala, E
Kubis, P
Rulka, J
[J]. MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE, 2002, 58 (04): : 300 - 302
[7] Evaluation of electrophoretic immunoblotting for the detection of antibodies against the bovine leukosis virus in cattle
Kittelberger, R
Laybourn, BJ
Diack, DS
Penrose, ME
Reichel, MP
Motha, J
Molloy, JB
Merza, M
[J]. JOURNAL OF VIROLOGICAL METHODS, 1996, 61 (1-2) : 7 - 22
[8] VALIDATION OF AN ENZYME-IMMUNOASSAY FOR DETECTION OF ANTIBODIES AGAINST BOVINE LEUKOSIS VIRUS
OLECHNOWITZ, AF
MIKO, A
KOEPERNIK, H
STARICK, E
FROBE, I
[J]. ARCHIV FUR EXPERIMENTELLE VETERINARMEDIZIN, 1990, 44 (02): : 279 - 288
[9] AN ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR ENZOOTIC BOVINE LEUKOSIS VIRUS-ANTIBODIES
TODD, D
ADAIR, BM
WIBBERLEY, G
[J]. VETERINARY RECORD, 1980, 107 (06) : 124 - 126
[10] ENZYME-LINKED-IMMUNO-SORBENT-ASSAY FOR DETECTION OF ANTIBODIES AGAINST BOVINE LEUCOSIS VIRUS
BEHRENS, F
ZIEGELMAIER, R
TOTH, T
KEYSERLINGK, MV
FORSCHNER, E
[J]. BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT, 1979, 92 (22): : 429 - 432

← 1 2 3 4 5 →