ISOLATION AND CHARACTERISTICS OF EIGHT NOVEL POLYMORPHIC MICROSATELLITE LOCI IN LIPPIA ALBA (VERBENACEAE)

被引：6
|
作者：
Santos, Fernanda R. C. [1 ]
Lima, Paula F. [1 ]
Priolli, Regina H. G. [2 ]
Siqueira, Walter J. [1 ]
Colombo, Carlos A. [1 ]
机构：
[1] Inst Agron IAC, Ctr Pesquisa & Desenvolvimento Recursos Genet Veg, BR-13075630 Sao Paulo, Brazil
[2] Univ Sao Paulo, Dept Genet, BR-13400970 Sao Paulo, Brazil
来源：
AMERICAN JOURNAL OF BOTANY | 2012年 / 99卷 / 08期
基金：
巴西圣保罗研究基金会;
关键词：
essential oil; genetic diversity; Lippia alba; medicinal plant; simple sequence repeat markers; Verbenaceae;
D O I：
10.3732/ajb.1100578
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: A set of eight microsatellite (simple sequence repeat [SSR]) markers for Lippia alba, an important medicinal and cosmetic plant, was developed to aid studies of genetic diversity and to define efficient strategies for breeding programs. Methods and Results: Using a (CT)(8)- and (GT)(8)-enriched library, a total of 11 SSR loci were developed and optimized in L. alba. Of the 11 loci, eight were found to be polymorphic after screening 61 accessions from two populations. The parameters used to characterize loci were expected heterozygosity (H-e) and number of alleles. A total of 44 alleles were identified, with an average of 5.5 alleles per loci, which were moderately to highly informative according to H-e. Conclusions: These new SSR markers have potential for informing genetic diversity, allele mining, and mapping studies and will be used to generate information for breeding programs of L. alba
引用
收藏
页码：E301 / E303
页数：3
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