Aqueous Microwave-Assisted Solid-Phase Peptide Synthesis Using Fmoc Strategy. II. Racemization Studies and Water Based Synthesis of Cysteine-Containing Peptides

被引：0
|
作者：
Hojo, Keiko [1 ]
Shinozaki, Natsuki
Hara, Asaki
Onishi, Mare
Fukumori, Yoshinobu
Ichikawa, Hideki
机构：
[1] Kobe Gakuin Univ, Fac Pharmaceut Sci, Chuo Ku, Kobe, Hyogo 6508586, Japan
来源：
PROTEIN AND PEPTIDE LETTERS | 2013年 / 20卷 / 10期
关键词：
Cysteine; microwave-assisted synthesis; nanoparticles; racemization solid-phase peptide synthesis; synthesis in water; N-PROTECTING GROUP; ORGANIC-SYNTHESIS; TETRAFLUOROBORATE; ACIDS;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a microwave (MW)-assisted peptide synthesis using Fmoc-amino acid nanoparticles in water previously. It is an organic solvent-free, environmentally friendly method for peptide synthesis. In this study, we have investigated the racemization of cysteine during an aqueous based coupling reaction with MW irradiation. Under our MW-assisted protocol using WSCI and DMTMM, the coupling reaction can be performed with low levels of racemization of cysteine. We also demonstrated the synthesis of the nonapeptide oxytocin analogue, Cys(Acm)-Tyr-Ile-Gln-Asn-Cys(Acm)-Pro-Leu-Gly-NH2 using our water based MW-assisted protocol with Fmoc-amino acid nanoparticles.
引用
收藏
页码：1122 / 1128
页数：7
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