Occurrence of Culturable Bacteria Living in Micropropagated Black Locust Cultures (Robinia pseudoacacia L.)

被引:0
|
作者
Zaspel, I. [1 ]
Ulrich, A. [2 ]
Boine, B. [1 ]
Stauber, T. [1 ]
机构
[1] Johann Heinrich von Thunen Inst vTI, Bundesforschungsinst Landl Raume Wald & Fischerei, Inst Forstgenet Waldsieversdorf, Waldsieversdorf, Germany
[2] Inst Landscape Matter Dynam, Leibnitz Ctr Agr Landscape Res ZALF, D-15374 Muncheberg, Germany
关键词
Robinia pseudoacacia; endophytic bacteria; 16S rDNA; tissue culture;
D O I
暂无
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
The culturable bacteria living as associated and endophytic microorganisms in tissue cultures of black locust selected randomly from roots as well as shoots were characterized. During a ten week cultivation period, the population density increased by approximately 102 to 103, and showed the highest density in the root tissue by approximately 10(8) to 10(9) cfu g(-1) fresh weight. Sequencing of the 16S rRNA gene of 119 isolated strains revealed 20 different genotypes be-longing to six genera from the phyla Actinobacteria and Proteobacteria. The Actinobacteria which represented the majority of isolates were divided into up to 12 16S rDNA genotypes belonging to the genera Microbacterium and Curtobacterium. The Proteobacteria (8 genotypes) was comprised of the genera Sphingomonas (2 genotypes), Methylobacterium (I genotype), Acidovorax (1 genotype), and Dyella (4 genotypes).
引用
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页码:231 / 235
页数:5
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