Reversed-phase liquid chromatography on a microspherical carbon column at high temperature

被引：16
|
作者：
Yamaki, S [1 ]
Isobe, T [1 ]
Okuyama, T [1 ]
Shinoda, T [1 ]
机构：
[1] KANAGAWA DENT COLL,DEPT DENT BIOTECHNOL & BIOENGN,KANAGAWA 238,JAPAN
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 728卷 / 1-2期
关键词：
temperature effects; carbon columns; stationary phases; LC; peptides; amino acids;
D O I：
10.1016/0021-9673(95)01215-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The chromatographic conditions for the use of a spherical carbon column at high temperatures up to 160 degrees C are described. The column heating system and the eluate cooling system are also described. Under the conditions studied, it was found that the carbon column at elevated temperature was stable and could be applicable to any compounds that are durable under these conditions. The peptide bonds at this high temperature and at this acidity were still stable enough for analysis, but the indole residues showed poor stability.
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页码：189 / 194
页数：6
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