Immunocytochemical localization of 60-kDa heat shock protein in Vibrio cholerae

被引:3
|
作者
Taguchi, H
Yamaguchi, H
Yamamoto, T
Kamiya, S
机构
[1] Department of Microbiology, Kyorin University, School of Medicine, Mitaka, Tokyo
[2] Department of Microbiology, Kyorin University, School of Medicine, Mitaka, Tokyo 181
关键词
D O I
10.1016/S0934-8840(96)80002-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The immunocytochemical localization of the 60-kDa hear shock protein (HSP) in Vibrio cholerae strain 569B was studied by transmission electron microscopy using a combination with the antigen-specific monoclonal antibody (5C3) and immunogold labelling. The labelling with gold particles in V. cholerae detected 2 types; the gold particles were exclusively detected in the cytoplasm for one type and in the periplasmic space for another type, suggesting that the 60-kDa HSP of V. cholerae corresponding immunologically to Escherichia coli GroEL may translocate from the cytoplasm to the periplasmic space in the V. cholerae cell.
引用
收藏
页码:496 / 500
页数:5
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