Effect of the Expousre to Low Dose of Ionizing radiation on KAU Hospital Medical Stuff by Using Early Response of Biological Dosimetry

Cytogenetic and Comet analysis were performed in forty volunteer students and hospital workers who were chronically exposed to Low ionizing radiation from king Abdulaziz University Hospital (KAUH), Radiology Department and Faculty of Applied Medical Sciences, were enrolled and divided into three groups. Peripheral blood samples were collected by venipuncture in heparinized and EDTA tubes (BD vacationer, Becton Dickinson, NJ, USA) on 6 different times during a period of 3 months. Accumulated absorbed doses calculated for the radiation workers ranged from 9.5 to 209.4 mSv. The mean of chromosomal aberration (CA) frequencies demonstrated statistically significant differences between the mean frequencies of CA between staff, intern students and second year students. Dicentric chromosome was only found in one technician from workers group while the other two groups have shown no dicentric chromosomes at all. Mean values (+/- standard deviation of the mean) of comet tail moment were 7.44 +/- 2.35 for the staff worker group and 3.51 +/- 2.1 for the intern students group and 3.01 +/- 1.33 for second year students (control group). Difference between mean tail moments were statistically significant when comparison between the worker stuff group and second year student group (P<0.01, ANOVA) and also significant between staff group and intern students (p<0.01, ANOVA) while there is no significance between intern and second year student groups (p>0.05, ANOVA). The range of tail moment in exposed worker stuff was 5.21-12.53 and for the intern students was 2.99-5.31 and for the control second year student was 2.00-4.37. These results also indicate that occupation and occupation periods significantly contributed to the level of primary DNA damage as recorded by mean of alkaline comet assay and the relevance of conducting cytogenetic analysis in parallel to physical dosimetry in routine clinical setting [Refaat I. F. EL-Fayoumi, Mohammed H. Saiem Al-Dahr, and Salah M kamal. Effect of the Expousre to Low Dose of Ionizing radiation on KAU Hospital Medical Stuff by Using Early Response of Biological Dosimetry. Life Sci J 2012;9(4):3986-3994]. (ISSN:1097-8135). http://www.lifesciencesite.com. 594