Droplet-Array (DA) Sandwich Chip: A Versatile Platform for High-Throughput Cell Screening Based on Superhydrophobic-Superhydrophilic Micropatterning

被引：178
|
作者：
Popova, Anna A. [1 ]
Schillo, Sebastian M. [2 ]
Demir, Konstantin [1 ]
Ueda, Erica [1 ]
Nesterov-Mueller, A. [2 ]
Levkin, Pavel A. [1 ]
机构：
[1] Karlsruhe Inst Technol, Inst Toxicol & Genet, D-76344 Eggenstein Leopoldshafen, Germany
[2] Karlsruhe Inst Technol, Inst Microstruct Technol, D-76344 Eggenstein Leopoldshafen, Germany
来源：
ADVANCED MATERIALS | 2015年 / 27卷 / 35期
关键词：
droplet-array sandwich chips; high throughput screening; superhydrophobic-superhydrophilic micropatterning; MICROARRAYS; ASSAY; CULTURE; FACILE;
D O I：
10.1002/adma.201502115
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A droplet-array (DA) sandwich chip is a miniaturized platform for cell-based high-throughput screening. It is based on sandwiching of a glass slide with a preprinted library and a superhydrophobic-superhydrophilic pattern, which consists of thousands of simultaneously formed microdroplets containing cells. The DA sandwich chip allows for one-step cell seeding, simultaneous initiation of screening, and 1000 times less reagent consumption than a regular 96-well plate. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：5217 / 5222
页数：6
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