Association of polymorphism at Exon-1 of the alpha 1-antitrypsin gene with milk production traits in Sahiwal and Karan Fries Cattle

PCR-RFLP analysis of PCR products was carried out using Sph I/P ae 1 enzyme for 100 Sahiwal cattle and 115 Karan Fries cattle. In Sahiwal cattle, 448 bp exhibited three genotypes viz., AA (448), AB (448+313+135bp), BB (313+135 bp) with corresponding genotype frequencies of 0.20, 0.45, 0.35 and gene frequencies 0.42 (A) and 0.58 (B). In Karan Fries cattle, genotype frequencies were 0.29, 0.21, 0.50 respectively with corresponding gene frequencies 0.39 (A) and 0.61 (B). These genotypes of 448 bp are highly significant for FL305DMY, FLTMY, FL305DSNFY and non significant for FL305 DFY and FL305DPY in Sahiwal. In Karan Fries cattle these genotypes are highly significant for FL305DMY, FLTMY, FL305DSNFY and FL305DPY and non significant for FL305DFY. AB genotype was superior for FL305DMY, FLTMY, FL305DSNFY traits and AA genotype was superior for FL305DFY and BB genotype was superior for FL305DPY trait in Sahiwal cattle and In Karan Fries Cattle, AB genotype was superior for all traits.