Study on Accurate Determination of Volatile Fatty Acids in Rumen Fluid by Capillary Gas Chromatography

被引：0
|
作者：
Luo, Cheng [1 ]
Cai, Suyi [1 ]
Jia, Linyan [1 ]
Tang, Xiang [1 ]
Zhang, Ruinan [1 ]
Jia, Gang [1 ]
Li, Hua [1 ]
Tang, Jiayong [1 ]
Liu, Guangmang [1 ]
Wu, Caimei [1 ]
机构：
[1] Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr & Feedstuffs, Key Lab Anim Dis Resistance Nutr Sichuan Prov, Inst Anim Nutr,China Minist Agr, Chengdu 611130, Peoples R China
来源：
PROCEEDINGS OF THE 5TH INTERNATIONAL CONFERENCE ON INFORMATION ENGINEERING FOR MECHANICS AND MATERIALS | 2015年 / 21卷
关键词：
Capillary gas chromatography; Rumen fluid; Volatile fatty acids; Internal standard method;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
In order to obtain accurate results of volatile fatty acids (VFAs) in rumen fluid by capillary gas chromatography (GC), a pretreatment method was firstly conducted by dissolving VFAs in the methanol. An internal standard method (ISM) with crotonic acid as the internal standard was adopted in the detection. The results showed that the reproducibility of VFA (acetic acid, propanoic acid and butyric acid) was enhanced remarkably with methanol added as the solvent. The reproducibility was dependent on both of the VFA concentration and methanol amount. In conclusion, methanol solvent was more suitable than aqueous solvent for VFA accurate detection with ISM by capillary GC.
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页码：386 / 391
页数：6
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