A novel method for separation of Rickettsiales-like organism "Candidatus Xenohaliotis californiensis" from host abalone tissue

被引：10
|
作者：
Roberto, Cruz-Flores [1 ]
Jorge, Caceres-Martinez [1 ,2 ]
Rebeca, Vasquez-Yeomans [2 ]
机构：
[1] Ctr Invest Cient & Educ Super Ensenada, Ensenada 22860, Baja California, Mexico
[2] Inst Sanidad Acuicola AC, Ensenada 22880, Baja California, Mexico
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 115卷
关键词：
Abalone; Haliotis; Withering syndrome; Candidates Xenohaliotis californiensis; Rickettsiales; WITHERING SYNDROME; HALIOTIS-RUFESCENS; RED ABALONE; SPP;
D O I：
10.1016/j.mimet.2015.05.021
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Filtrations were applied to separate vacuoles of Rickettsiales-like organism Candidates Xenohaliotis californiensis. Vacuoles were visualized by staining with nucleic acid fluorochrome and their identity was confirmed by Laser Capture Microdissection and PCR. This methodology separates vacuoles and allows studies without the need of isolation in synthetic media or cell lines. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：79 / 82
页数：4
相关论文
共 14 条
  • [1] Coexistence of the stippled Rickettsiales-like prokaryote with Candidatus Xenohaliotis californiensis and the bacteriophage pCXc in farmed red abalone Haliotis rufescens from Mexico
    Caceres-Martinez, Jorge
    Cruz-Flores, Roberto
    Vasquez-Yeomans, Rebeca
    Tinoco-Orta, Gissel
    AQUACULTURE, 2021, 532
  • [2] The hyperparasite of the rickettsiales-like prokaryote, Candidatus Xenohaliotis californiensis has morphological characteristics of a Siphoviridae (Caudovirales)
    Cruz-Flores, Roberto
    Caceres-Martinez, Jorge
    JOURNAL OF INVERTEBRATE PATHOLOGY, 2016, 133 : 8 - 11
  • [3] Transmission of the Rickettsiales-like prokaryote "Candidatus xenohaliotis californiensis" and its role in Withering syndrome of California abalone, Haliotis spp.
    Moore, JD
    Robbins, TT
    Hedrick, RP
    Friedman, CS
    JOURNAL OF SHELLFISH RESEARCH, 2001, 20 (02): : 867 - 874
  • [4] Detection of 'Candidatus Xenohaliotis californiensis' (Rickettsiales-like prokaryote) inclusions in tissue squashes of abalone (Haliotis spp.) gastrointestinal epithelium using a nucleic acid fluorochrome
    Moore, JD
    Cherr, GN
    Friedman, CS
    DISEASES OF AQUATIC ORGANISMS, 2001, 46 (02) : 147 - 152
  • [5] Hyperparasitism by the bacteriophage (Caudovirales) infecting Candidatus Xenohaliotis californiensis (Rickettsiales-like prokaryote) parasite of wild abalone Haliotis fulgens and Haliotis corrugata from the Peninsula of Baja California, Mexico
    Cruz-Flores, Roberto
    Caceres-Martinez, Jorge
    Munoz-Flores, Monserrat
    Vasquez-Yeomans, Rebeca
    Hernandez Rodriguez, Monica
    Del Rio-Portilla, Miguel Angel
    Rocha-Olivares, Axayacatl
    Castro-Longoria, Ernestina
    JOURNAL OF INVERTEBRATE PATHOLOGY, 2016, 140 : 58 - 67
  • [6] Monomorphic pathogens: The case of Candidatus Xenohaliotis californiensis from abalone in California, USA and Baja California, Mexico
    Cicala, Francesco
    Moore, James D.
    Caceres-Martinez, Jorge
    Del Rio-Portilla, Miguel A.
    Hernandez-Rodriguez, Monica
    Vasquez-Yeomans, Rebeca
    Rocha-Olivares, Axayacatl
    JOURNAL OF INVERTEBRATE PATHOLOGY, 2018, 154 : 19 - 23
  • [7] Rapid and Simple Detection Method of "Candidatus Xenohaliotis californiensis" Using Fecal PCR in Abalone Haliotis discus discus and H. gigantea
    Kiryu, Ikunari
    Nishioka, Toyohiro
    Yuasa, Kei
    Kurita, Jun
    Shimahara, Yoshiko
    Ototake, Mitsuru
    Ikegami, Naoya
    Oseko, Norihisa
    FISH PATHOLOGY, 2014, 49 (02): : 41 - 48
  • [8] ISOLATION OF A RICKETTSIALES-LIKE ORGANISM FROM DISEASED COHO SALMON (ONCORHYNCHUS-KISUTCH) IN CHILE
    FRYER, JL
    LANNAN, CN
    GARCES, LH
    LARENAS, JJ
    SMITH, PA
    FISH PATHOLOGY, 1990, 25 (02): : 107 - 114
  • [9] Metagenome-assembled genome of withering syndrome causative agent, "Candidatus Xenohaliotis californiensis," from endangered white abalone (Haliotis sorenseni)
    Kunselman, Emily
    Allard, Sarah
    Burge, Colleen
    Marshman, Blythe
    Frederick, Alyssa
    Gilbert, Jack
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2024, 13 (06):
  • [10] Prevalence and intensity of a Rickettsiales-like organism in cultured pleasure oyster, Crassostrea corteziensis , from Nayarit, Mexico
    Martinez, Teresita Del Carmen Guarneros
    Caceres-Martinez, Jorge C.
    -Flores, Roberto Cruz
    Lopez-Carvallo, Jesus Antonio
    Del Rio-Portilla, Miguel Angel
    Renteria, Yanet Guerrero
    JOURNAL OF INVERTEBRATE PATHOLOGY, 2024, 204
12