A rapid differentiation method for enteroinvasive Escherichia coli

被引:3
|
作者
Aribam, Swarmistha Devi [1 ]
Hirota, Jiro [1 ]
Kusumoto, Masahiro [1 ]
Harada, Tomoyuki [1 ]
Shiraiwa, Kazumasa [1 ]
Ogawa, Yohsuke [1 ]
Shimoji, Yoshihiro [1 ]
Eguchi, Masahiro [1 ]
机构
[1] NARO, Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan
关键词
Enteroinvasive Escherichia coli; Rapid detection; Gentamicin invasion assay; SHIGELLA; STRAINS; EIEC;
D O I
10.1016/j.mimet.2013.11.012
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Enteroinvasive Escherichia coli (EIEC) comprise 21 major serotypes defined by the presence of O and H antigens, and diagnosis depends on determining its invasive potential. Using HEp-2 cells infected with an EIEC strain, we developed a simple growth-dependent assay that differentiated EIEC strain from non-invasive strains 6 h after infection. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:64 / 66
页数:3
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