Novel method for evaluation of the oligomeric structure of membrane proteins

被引：122

作者：

Ramjeesingh, M ^{[1
]}

Huan, LJ ^{[1
]}

Garami, E ^{[1
]}

Bear, CE ^{[1
]}

机构：

[1] Hosp Sick Children, Res Inst, Dept Cell Biol, Toronto, ON M5G 1X8, Canada

来源：

BIOCHEMICAL JOURNAL | 1999年 / 342卷

关键词：

ion channels; multimeric complexes; quaternary structure; SDS/PAGE;

D O I：

10.1042/0264-6021:3420119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Assessment of the quaternary structure of membrane proteins by PAGE has been problematic owing to their relatively poor solubility in non-dissociative detergents. Here we report that several membrane proteins can be readily solubilized in their native quaternary structure with the use of the detergent perfluoro-octanoic acid (PFO). Further, PFO can be used with PAGE, thereby providing a novel, accessible tool with which to assess the molecular mass of homo-multimeric protein complexes.

引用

页码：119 / 123

页数：5

共 50 条

[1] EVALUATION OF COMPLEX-STOICHIOMETRY OF OLIGOMERIC MEMBRANE-PROTEINS BY DIRECT EDMAN DEGRADATION
BUSE, G
JOURNAL OF PROTEIN CHEMISTRY, 1988, 7 (03): : 203 - 203
[2] Oligomeric structure, dynamics, and orientation of membrane proteins from solid-state NMR
Hong, Mei
STRUCTURE, 2006, 14 (12) : 1731 - 1740
[3] Accurate Determination of Conformational Transitions in Oligomeric Membrane Proteins
Máximo Sanz-Hernández
Vitaly V. Vostrikov
Gianluigi Veglia
Alfonso De Simone
Scientific Reports, 6
[4] BIOSYNTHESIS AND SURFACE EXPRESSION OF OLIGOMERIC MEMBRANE-PROTEINS
DOBBERSTEIN, B
KVIST, S
BURGERT, HG
BIOLOGY OF THE CELL, 1982, 45 : S357 - S357
[5] Determination of the oligomeric assembly of integral membrane proteins.
Eskandari, S
Kreman, M
Wright, EM
Zampighi, GA
FASEB JOURNAL, 1998, 12 (05): : A1013 - A1013
[6] Accurate Determination of Conformational Transitions in Oligomeric Membrane Proteins
Sanz-Hernandez, Maximo
Vostrikov, Vitaly V.
Veglia, Gianluigi
De Simone, Alfonso
SCIENTIFIC REPORTS, 2016, 6
[7] A NOVEL METHOD FOR SPECIFIC RADIOLABELING OF MEMBRANE-PROTEINS
LAU, AL
THOMPSON, JA
CUNNINGHAM, DD
JOURNAL OF CELLULAR BIOCHEMISTRY, 1986, : 250 - 250
[8] Systematic Evaluation of the CS-Rosetta De Novo Structure Prediction Method for Membrane Proteins
Reichel, Katrin
Fisette, Olivier
Braun, Tatjana
Hummer, Gerhard
Lange, Oliver
Schaefer, Lars
BIOPHYSICAL JOURNAL, 2016, 110 (03) : 39A - 39A
[9] MEMBRANE PROTEINS AND MEMBRANE STRUCTURE
CAPALDI, RA
GREEN, DE
FEBS LETTERS, 1972, 25 (02) : 205 - &
[10] Oligomeric States and Cooperative Gating in Clusters of Mechanosensitive Membrane Proteins
Kahraman, Osman
Klug, William S.
Haselwandter, Christoph A.
BIOPHYSICAL JOURNAL, 2014, 106 (02) : 299A - 299A

← 1 2 3 4 5 →