Functional evidence for the involvement of Arabidopsis IspF homolog in the nonmevalonate pathway of plastid isoprenoid biosynthesis

被引:48
|
作者
Hsieh, MH [1 ]
Goodman, HM
机构
[1] Acad Sinica, Inst Plant & Microbial Biol, Taipei 11529, Taiwan
[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA
[3] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
关键词
albino; Arabidopsis; chloroplast; isoprenoids; IspF; nonmevalonate pathway;
D O I
10.1007/s00425-005-0140-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
There are two independent pathways, the cytosolic mevalonate (MVA) pathway and the plastid nonmevalonate (nonMVA) pathway, to synthesize isopentenyl diphosphate and dimethylallyl diphosphate in plants. Carotenoids and the phytyl side chain of chlorophylls are isoprenoids derived from the plastid nonMVA pathway. All enzymes involved in the nonMVA pathway have been identified in Escherichia coli. The E. coli IspF protein catalyzes a unique cyclization reaction to convert 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate in the nonMVA pathway. We have characterized an Arabidopsis T-DNA insertion mutant, ispF-1, which has a null mutation in the IspF gene. Homozygous ispF-1 mutants are albino lethal and the IspF transcripts are undetectable in these plants. Moreover, the ispF-1 mutant chloroplasts are filled with vesicles instead of thylakoids. Amino acid sequence alignment reveals that the IspF proteins are highly conserved between plants and bacteria. Interestingly, expression of the Arabidopsis IspF protein can rescue the lethal phenotype of an E. coli ispF Mutant. These results indicate that the Arabidopsis IspF may share similar enzymatic mechanisms with the E. coli protein.
引用
收藏
页码:779 / 784
页数:6
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