Detection of Escherichia coli O104 in the Feces of Feedlot Cattle by a Multiplex PCR Assay Designed To Target Major Genetic Traits of the Virulent Hybrid Strain Responsible for the 2011 German Outbreak

被引：23

作者：

Paddock, Z. D. ^{[1
]}

Bai, J. ^{[2
]}

Shi, X. ^{[1
]}

Renter, D. G. ^{[1
]}

Nagaraja, T. G. ^{[1
]}

机构：

[1] Kansas State Univ, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA

[2] Kansas State Univ, Vet Diagnost Lab, Manhattan, KS 66506 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2013年 / 79卷 / 11期

基金：

美国食品与农业研究所;

关键词：

HEMOLYTIC-UREMIC SYNDROME; O157; SEROGROUPS; O26; O145; O111; APPLICABILITY; O121; O45;

D O I：

10.1128/AEM.00246-13

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multiplex PCR was designed to detect Escherichia coli O104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregative E. coli, in cattle feces. A total of 248 fecal samples were tested, and 20.6% were positive for serogroup O104. The O104 isolates did not carry genes characteristic of the virulent hybrid strain.

引用

页码：3522 / 3525

页数：4

共 4 条

[1] Identification of the Shiga Toxin-Producing Escherichia coli O104:H4 Strain Responsible for a Food Poisoning Outbreak in Germany by PCR
Qin, Junjie
Cui, Yujun
Zhao, Xiangna
Rohde, Holger
Liang, Ting
Wolters, Manuel
Li, Dongfang
Campos, Cristina Belmar
Christner, Martin
Song, Yajun
Yang, Ruifu
JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (09) : 3439 - 3440
[2] Multiplex Quantitative PCR Assays for the Detection and Quantification of the Six Major Non-O157 Escherichia coli Serogroups in Cattle Feces
Shridhar, P. B.
Noll, L. W.
Shi, X.
An, B.
Cernicchiaro, N.
Renter, D. G.
Nagaraja, T. G.
Bai, J.
JOURNAL OF FOOD PROTECTION, 2016, 79 (01) : 66 - 74
[3] An imported case of bloody diarrhea in the Czech Republic caused by a hybrid enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104:H4 strain associated with the large outbreak in Germany, May 2011
Marejkova, M.
Rohacova, H.
Reisingerova, M.
Petras, P.
FOLIA MICROBIOLOGICA, 2012, 57 (02) : 85 - 89
[4] An imported case of bloody diarrhea in the Czech Republic caused by a hybrid enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104:H4 strain associated with the large outbreak in Germany, May 2011
M. Marejková
H. Roháčová
M. Reisingerová
P. Petráš
Folia Microbiologica, 2012, 57 : 85 - 89

← 1 →