A Simple Mechanism Based on Amino Acid Substitutions is not a Critical Determinant of High Mortality of Japanese Encephalitis Virus Infection in Mice

被引:1
|
作者
Takamatsu, Yuki [1 ,4 ]
Uchida, Leo [1 ,5 ]
Raekiansyah, Muhareva [1 ]
Luz, Mark Anthony [1 ]
Morita, Kouichi [1 ,2 ]
Hayasaka, Daisuke [1 ,2 ,3 ]
机构
[1] Nagasaki Univ, Inst Trop Med, Dept Virol, 1-12-4 Sakamoto, Nagasaki 8528523, Japan
[2] Nagasaki Univ, Leading Grad Sch Program, Nagasaki 8528523, Japan
[3] Nagasaki Univ, Ctr Control & Prevent Infect Dis, Nagasaki 8528523, Japan
[4] Philipps Univ Marburg, Inst Virol, D-35037 Marburg, Germany
[5] Rakuno Gakuen Univ, Lab Zoonot Dis, Div Hlth & Environm Sci, Sch Vet Med, Ebetsu, Hokkaido 0698501, Japan
来源
VIRUSES-BASEL | 2018年 / 10卷 / 02期
基金
日本学术振兴会;
关键词
Japanese encephalitis virus; pathogenicity; mouse; recombinant; amino acid substitution; IN-VITRO; PROTEIN; ATTENUATION; NEUROVIRULENCE; NUCLEOTIDE; FLAVIVIRUS; STRAIN; CELLS; NS1'; NS2A;
D O I
10.3390/v10020062
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
For the development of effective treatment strategies for Japanese encephalitis (JE), it is important to identify the viral factors causing severe disease during JE virus (JEV) infection. In this study, we assessed whether amino acid substitutions are critical factors for higher mortality of JaTH160 compared with JaOArS982 in mice using the technique of infectious cDNA clones. We raised the possibility that two amino acids of C-124 and NS3(482) of JaTH160 may contribute to increased mortality in mice. However, simultaneous substitutions of these amino acids did not significantly increase the virulence of JaOArS982, suggesting that high mortality due to JaTH160 viral infection cannot be simply attributed to the specific amino acids. Multiple and complex, but not simple, mechanisms may induce the high mortality of JaTH160 infection in mice.
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页数:10
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