PEG-based ultrasound-assisted enzymatic extraction of polysaccharides from Ginkgo biloba leaves

被引：36
|
作者：
Zhang, Lijin [1 ]
Guo, Shanshan [1 ]
Wang, Maoshan [1 ]
He, Lei [1 ]
机构：
[1] Univ Jinan, Sch Hotel Management, Dept Food Sci & Nutr, Jinan 250002, Shandong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 80卷
关键词：
Ginkgo biloba leaves; Polysaccharides; Polyethylene glycol-based ultrasound-assisted enzymatic extraction; Response surface methodology; Box-Behnken design; RESPONSE-SURFACE METHODOLOGY; BOX-BEHNKEN DESIGN; ANTIOXIDANT ACTIVITY; POLYETHYLENE-GLYCOL; IN-VITRO; CRUDE POLYSACCHARIDES; L; LEAVES; OPTIMIZATION; SOLVENT; FRUIT;
D O I：
10.1016/j.ijbiomac.2015.07.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, one kind of environmentally friendly solvents named polyethylene glycol (PEG) was developed for the ultrasound-assisted enzymatic extraction (UAEE) of polysaccharides from Gingko biloba leaves (GBLP). Box-Behnken design (BBD) was used to optimize the UAEE conditions of GBLP. Results showed that the optimal extraction conditions were: a pH of 4.34, an extraction temperature of 51.88 degrees C and an extraction time of 37.13 min. Under these optimal extraction conditions, the GBLP yield was 7.29 +/- 0.21%, which was well in agreement with the value predicted by the mathematical model. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：644 / 650
页数：7
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