The Arabidopsis TAC Position Viewer: a high-resolution map of transformation-competent artificial chromosome (TAC) clones aligned with the Arabidopsis thaliana Columbia-0 genome

被引：3

作者：

Hirose, Yoshitsugu ^{[1
]}

Suda, Kunihiro ^{[2
]}

Liu, Yao-Guang ^{[3
,4
]}

Sato, Shusei ^{[2
,5
]}

Nakamura, Yukino ^{[6
]}

Yokoyama, Koji ^{[6
]}

Yamamoto, Naoki ^{[2
,6
]}

Hanano, Shigeru ^{[2
]}

Takita, Eiji ^{[2
]}

Sakurai, Nozomu ^{[2
]}

Suzuki, Hideyuki ^{[2
]}

Nakamura, Yasukazu ^{[2
]}

Kaneko, Takakazu ^{[2
]}

Yano, Kentaro ^{[6
]}

Tabata, Satoshi ^{[2
]}

Shibata, Daisuke ^{[2
]}

机构：

[1] Honda Res Inst Japan Co Ltd, Wako, Saitama 3510188, Japan

[2] Kazusa DNA Res Inst, Chiba 2920818, Japan

[3] South China Agr Univ, Genet Engn Lab, Gungzhou 510642, Peoples R China

[4] South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Gungzhou 510642, Peoples R China

[5] Tohoku Univ, Grad Sch Life Sci, Aoba Ku, Sendai, Miyagi 9808577, Japan

[6] Meiji Univ, Sch Agr, Dept Life Sci, Bioinformat Lab,Tama Ku, Kawasaki, Kanagawa 2148571, Japan

来源：

PLANT JOURNAL | 2015年 / 83卷 / 06期

关键词：

Arabidopsis thaliana; transformation-competent artificial chromosome; Arabidopsis TAC clones; Arabidopsis TAC library; Arabidopsis genome; genomics; bioinformatics; GBrowse; INFORMATION RESOURCE TAIR; SYSTEMS ANALYSES; PLANT FUNCTIONS; FLOWERING TIME; DNA FRAGMENTS; PHYSICAL MAP; GENE; CLONING; BIOINFORMATICS; ANNOTATION;

D O I：

10.1111/tpj.12949

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We present a high-resolution map of genomic transformation-competent artificial chromosome (TAC) clones extending over all Arabidopsis thaliana (Arabidopsis) chromosomes. The Arabidopsis genomic TAC clones have been valuable genetic tools. Previously, we constructed an Arabidopsis genomic TAC library consisting of more than 10000 TAC clones harboring large genomic DNA fragments extending over the whole Arabidopsis genome. Here, we determined 13577 end sequences from 6987 Arabidopsis TAC clones and mapped 5937 TAC clones to precise locations, covering approximately 90% of the Arabidopsis chromosomes. We present the large-scale data set of TAC clones with high-resolution mapping information as a Java application tool, the Arabidopsis TAC Position Viewer, which provides ready-to-go transformable genomic DNA clones corresponding to certain loci on Arabidopsis chromosomes. The TAC clone resources will accelerate genomic DNA cloning, positional walking, complementation of mutants and DNA transformation for heterologous gene expression.

引用

页码：1114 / 1122

页数：9