A Novel Model of the Action Potential of Ventricular-like Human Induced Pluripotent Stem Cell-derived Cardiomyocytes

Human induced pluripotent stem cells (hiPSCs) represent nowadays a valuable in-vitro model to study the mechanisms underlying pathologies and focusing on drug treatment in a patient-specific manner. In-silico models can integrate the experimental practice being used as simulation platforms, providing new hints and helping defining new experiments and hypothesis. We developed a new model of ventricular-like hiPSC-derived cardiomyocyte (hiPSC-CM) based on recently published data and aiming to provide a detailed description of the hiPSC-CM electrophysiology. Our model reproduced: (i) spontaneous action potentials (APs); (ii) AP features typical of the ventricular-like phenotype such as maximum diastolic potential, AP duration and amplitude; (iii) effects of prototypical current blockers. In conclusion our new hiPSC-CM model represents a validated description of the electrophysiology of ventricular-like hiPSC-CM and it has potential application in further studies on patient-and disease-specific ion channels mutations in hiPS-CMs.