Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles

被引：0
|
作者：
Hema, M. [1 ]
Reddy, Ch V. Subba [1 ]
Savithri, H. S. [2 ]
Sreenivasulu, P. [1 ]
机构：
[1] Sri Venkateswara Univ, Dept Virol, Coll Biol & Earth Sci, Tirupati 517502, Andhra Pradesh, India
[2] Indian Inst Sci, Dept Biochem, Bangalore 526012, Karnataka, India
来源：
INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY | 2008年 / 46卷 / 11期
关键词：
Potyvirus-like particles; Recombinant coat protein; Sugarcane streak mosaic virus;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.
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页码：793 / 796
页数：4
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