Determination of the total Ginsenosides in ginseng using the UV Spectrophotometer and Evaluation of the Measurement Uncertainty

Using the Ultraviolet Visible Spectroscopical Photometry method, the Ginsenosides content was detected, and evaluated the measuring uncertainty of the Ginsenosides content, analyzed the main source of the uncertainty components, finally evaluated and calculated the uncertainty components. The results showed that, when the Ginsenosides content is in the range of 20-120 mu g, there is a good linear relation, and the linear equation is A=0.0216+0.00556C, r=0.9961 (coefficient correlation). For example, when the content of Ginsenosides is 4.60%, the expanded uncertainty of Ginsenosides measurement is U=0.408%, the coverage factor is 2 (k=2). This study provides a reliable theoretical basis for an effective and high quality method of Ginsenosides content measurement.