The Interactions of the Largest Subunit of RNA Polymerase II with Other Cellular Proteins: a Bioinformatic Approach

被引：0

作者：

Shukla, Abhijit ^{[1
]}

Natarajan, Aparna ^{[1
]}

Bhaumik, Sukesh ^{[1
]}

El-Shemy, Hany A. ^{[2
,3
]}

Lightfoot, David ^{[1
]}

机构：

[1] So Illinois Univ, Sch Med, Dept Biochem & Mol Biol, Carbondale, IL 62901 USA

[2] Cairo Univ, FARP, Giza 12613, Egypt

[3] Cairo Univ, Fac Agr, Dept Biochem, Giza 12613, Egypt

来源：

CURRENT ISSUES IN MOLECULAR BIOLOGY | 2009年 / 11卷

关键词：

bioinformatics; protein interactions; Rbp1; yeast; human; CHROMATIN-REMODELING COMPLEX; STRAND BREAK REPAIR; SACCHAROMYCES-CEREVISIAE; MESSENGER-RNA; TRANSCRIPTIONAL ELONGATION; HISTONE H3; 3'-END FORMATION; GENE-EXPRESSION; PAF1; COMPLEX; IN-VIVO;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The function of a protein is governed by its interaction with other proteins inside a cell. Therefore, it is important to identify the interacting partners of a particular protein to decipher its function. The protein interaction networks are generally determined by bioinformatic as well as experimental methodologies such as yeast two hybrid, mass spectrometry, immunoprecipitation, and fluorescence resonance energy transfer assays. Here, we have analyzed bioinformatically the interactions of Rpb1p (the largest subunit of RNA Polymerase II) with other proteins in yeast, using Cytoscape software and Biogrid/Biomart database. We find that Rpb1p interacts with a large number of proteins involved in mRNA synthesis, processing, export, and other cellular processes. These results validate the application of such bioinformatic approach to determine the interactome for other cellular proteins.

引用

页码：I65 / i71

页数：7

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