Inhibition of hepatitis B virus replication by small interference RNA induces expression of MICA in HepG2.2.15 cells

被引：19

作者：

Tang, Kai-Fu ^{[1
]}

Chen, Min ^{[1
]}

Xie, Jing ^{[1
]}

Song, Guan-Bin ^{[2
]}

Shi, Yi-Song ^{[2
]}

Liu, Qi ^{[1
]}

Mei, Zhe-Chuan ^{[3
]}

Steinle, Alexander ^{[4
]}

Ren, Hong ^{[1
]}

机构：

[1] Chongqing Univ Med Sci, Inst Viral Hepatitis, Affiliated Hosp 2, Key Lab Mol Biol Infect Dis,State Minist Educ, Chongqing 400010, Peoples R China

[2] Chongqing Univ, Coll Bioengn, Key Lab Biomech & Tissue Engn, State Minist Educ, Chongqing 400044, Peoples R China

[3] Chongqing Univ Med Sci, Dept Gastroenterol, Affiliated Hosp 2, Chongqing 400010, Peoples R China

[4] Univ Tubingen, Inst Cell Biol, Dept Immunol, D-72076 Tubingen, Germany

来源：

MEDICAL MICROBIOLOGY AND IMMUNOLOGY | 2009年 / 198卷 / 01期

基金：

中国国家自然科学基金;

关键词：

MICA; NKG2D; HBV; Small interference RNA; Hepatocellular carcinoma; HEPATOCELLULAR-CARCINOMA; T-CELLS; LAMIVUDINE TREATMENT; NKG2D; LIGANDS; DNA; IMMUNORECEPTOR; INFECTION; THERAPY;

D O I：

10.1007/s00430-008-0101-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Hepatitis B virus (HBV) replicates in most tumor tissues of patients with HBV-associated hepatocellular carcinoma (HCC). In the present study, we have shown that the expression of HBV in the HCC cell lines, HepG2 and Huh7, down-regulated the expression of MHC class I-related molecule A (MICA), a ligand of the NKG2D receptor. Inhibition of HBV expression by small interference RNAs (siRNAs) in HepG2.2.15, a cell line that constitutively expresses HBV, induced up-regulation of MICA. The up-regulation of MICA increased the lysis of HepG2.2.15 cells by NK cells. Our results suggest that HBV compromises the innate immune system in HCC patients and that inhibition of HBV replication by siRNAs may enhance the antitumor immune response.

引用

页码：27 / 32

页数：6

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