Use of pyranose oxidase enzyme in inhibitor biosensing

被引：5
|
作者：
Yazgan, Idris [1 ]
Aydin, Tuba [1 ]
Odaci, Dilek [1 ]
Timur, Suna [1 ]
机构：
[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey
来源：
ANALYTICAL LETTERS | 2008年 / 41卷 / 11期
关键词：
ethanol; glutathione (GSH); inhibitor based biosensor; pyranose oxidase (PyOx);
D O I：
10.1080/00032710802209276
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An inhibition based biosensing system was developed for the determination of glutathione (GSH) and ethanol (EtOH) as pyranose oxidase (PyOx) inhibitors. The PyOx was immobilized in carbon paste electrode and the amperometric detection of hydrogen peroxide through the enzymatic reaction was monitored at+0.9V versus Ag/AgCl. In addition to the optimization studies, analytical characteristics and the effect of various compounds on the biosensor response were researched. Finally, the proposed system was applied to analyze GSH and EtOH in real matrices.
引用
收藏
页码：2088 / 2096
页数：9
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