Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

被引：10
|
作者：
da Silva, Marcel Fernando [1 ]
Goncalves, Marcos Cesar [2 ]
Brito, Michael dos Santos [3 ]
Nobile, Paula Macedo [1 ]
de Andrade, Larissa Mara [1 ]
Medeiros, Cibele Nataliane [1 ]
Creste, Silvana [1 ]
Pinto, Luciana Rossini [1 ]
机构：
[1] Ctr Cana, Inst Agron, CP 206, BR-14001970 Ribeirao Preto, SP, Brazil
[2] Crop Protect Res Ctr, Inst Biol, Sao Paulo, SP, Brazil
[3] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Sao Jose Dos Campos, SP, Brazil
来源：
BMC RESEARCH NOTES | 2019年 / 12卷 / 1期
基金：
巴西圣保罗研究基金会;
关键词：
BestKeeper; GeNorm; Normalization; NormFinder; RefFinder; Saccharum spp; Sugarcane mosaic disease;
D O I：
10.1186/s13104-019-4168-5
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Objective: The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome analysis. In this regard, seven potential reference genes were tested by RT-qPCR and ranked according to their stability using BestKeeper, NormFinder and GeNorm algorithms, and RefFinder WEB-based software in an experiment performed with samples from two sugarcane cultivars contrasting for SCMV resistance, when mechanically inoculated with a severe SCMV strain and using mock inoculated plant controls. Results: The genes Uridylate kinase (UK) and Ubiquitin-conjugating enzyme 18 (UBC18) were the most stable according to GeNorm algorithm and the Pearson correlation coefficients with the BestKeeper index. On the other hand, ribosomal protein L35-4 (RPL1), Actin (ACT) and Ubiquitin1 (UBQ1) were the least stable genes for all algorithms tested.
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页数：7
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