MTV1 and MTV4 Encode Plant-Specific ENTH and ARF GAP Proteins That Mediate Clathrin-Dependent Trafficking of Vacuolar Cargo from the Trans-Golgi Network

被引：50

作者：

Sauer, Michael ^{[1
]}

Delgadillo, Am. Otilia ^{[1
]}

Zouhar, Jan ^{[1
,2
]}

Reynolds, Gregory D. ^{[3
]}

Pennington, Janice G. ^{[4
]}

Jiang, Liwen ^{[5
]}

Liljegren, Sarah J. ^{[6
]}

Stierhof, York-Dieter ^{[7
]}

De Jaeger, Geert ^{[8
,9
]}

Otegui, Marisa S. ^{[4
]}

Bednarek, Sebastian Y. ^{[3
]}

Rojo, Enrique ^{[1
]}

机构：

[1] CSIC, Centro Nacl Biotecnol, Dept Mol Plantas, Madrid 28049, Spain

[2] Univ Politecn Madrid, Ctr Biotecnol & Genom Plantas, Madrid 28223, Spain

[3] Univ Madison, Dept Biochem, Madison, WI 53706 USA

[4] Univ Madison, Dept Bot, Madison, WI 53706 USA

[5] Chinese Univ Hong Kong, Ctr Cell & Dev Biol, Sch Life Sci, New Territories, Hong Kong, Peoples R China

[6] Univ Mississippi, Dept Biol, Oxford, MS 38677 USA

[7] Univ Tubingen, Zentrum Mol Biol Pflanzen, D-72076 Tubingen, Germany

[8] VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[9] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium

来源：

PLANT CELL | 2013年 / 25卷 / 06期

基金：

美国国家科学基金会;

关键词：

TANDEM AFFINITY PURIFICATION; GTPASE-ACTIVATING PROTEIN; SEED STORAGE PROTEINS; ARABIDOPSIS-THALIANA; SORTING RECEPTOR; ENDOPLASMIC-RETICULUM; MULTIVESICULAR BODIES; COATED VESICLES; INTRACELLULAR TRAFFICKING; PREVACUOLAR COMPARTMENTS;

D O I：

10.1105/tpc.113.111724

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. In particular, it is unknown whether clathrin-coated vesicles (CCVs) participate in this transport step. Through a screen for modified transport to the vacuole (mtv) mutants that secrete the vacuolar protein VAC2, we identified MTV1, which encodes an EPSIN N-TERMINAL HOMOLOGY protein, and MTV4, which encodes the ADP ribosylation factor GTPase-activating protein NEVERSHED/AGD5. MTV1 and NEV/AGD5 have overlapping expression patterns and interact genetically to transport vacuolar cargo and promote plant growth, but they have no apparent roles in protein secretion or endocytosis. MTV1 and NEV/AGD5 colocalize with clathrin at the TGN and are incorporated into CCVs. Importantly, mtv1 nev/agd5 double mutants show altered subcellular distribution of CCV cargo exported from the TGN. Moreover, MTV1 binds clathrin in vitro, and NEV/AGD5 associates in vivo with clathrin, directly linking these proteins to CCV formation. These results indicate that MTV1 and NEV/AGD5 are key effectors for CCV-mediated trafficking of vacuolar proteins from the TGN to the PVC in plants.

引用

页码：2217 / 2235

页数：19