Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

被引：53
|
作者：
Meyer, S. [1 ,2 ]
Lopez-Serrano, A. [3 ]
Mitze, H. [1 ]
Jakubowski, N. [3 ]
Schwerdtle, T. [1 ,2 ,4 ]
机构：
[1] Univ Potsdam, Inst Nutr Sci, Arthur Scheunert Allee 114-116, D-14558 Nuthetal, Germany
[2] NutriAct Competence Cluster Nutr Res Berlin Potsd, Nuthetal, Germany
[3] Bundesanstalt Mat Forsch & Prufung BAM, Richard Willstatter Str 11, D-12489 Berlin, Germany
[4] DFG Res Unit, Interact Essential Trace Elements Hlth & Dis Elde, FOR 2558, Nuthetal, Germany
来源：
METALLOMICS | 2018年 / 10卷 / 01期
关键词：
MASS-SPECTROMETRY; QUANTIFICATION; NANOPARTICLES;
D O I：
10.1039/c7mt00285h
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 mu M for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.
引用
收藏
页码：73 / 76
页数：4
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