Development and Validation of a Reverse Phase HPLC Method for the Estimation of Lacidipine in Nanoformulations

The objective of the present study was to develop and validate a simple, accurate and reliable reverse phase high-performance liquid chromatography method for the estimation of lacidipine. The method was developed on Grace Vydac RP C-18 Column (250 x 4.6 mm i.d., particle size 5 mu m) using mobile phase consisting in the ratio of 90: 10 acetonitrile: 0.1% triethylamine (TEA) solution (pH 6.5). The flow rate was maintained at 0.8 mL/min and effluent was monitored at 240 nm using UV detector. The retention time was found to be 7.4 min. Calibration curve was linear over the concentration range of 0.5-10 mu g/mL. Intra-and inter-day precision % RSD values were found to be 0.541 and 0.828%, respectively. Recovery of lacidipine was in the range of 100.223-101.046 %. The limits of detection and quantification were 29.28 ng/mL and 88.73 ng/mL, respectively. The developed method was successfully applied for the quantitative determination of lacidipine in solid lipid nanoparticles.