Multivariate morphometric differentiation between females of two cryptic species of Lutzomyia subgenus Helcocyrtomyia (Diptera: Psychodidae)

The vectorial capacity of sibling species of the Neotropical genus Lutzomyia is likely to differ, thus a means of identifying the most important vector species is of critical importance to the epidemiology and control of the leishmanioses. Multivariate statistical procedures were employed to determine whether the females of two sibling sandfly species (genus Lutzomyia) of the subgenus Helcocyrtomyia, L. ceferinoi (N = 31) and L. erwindonaldoi (N = 32), can be discriminated oil the basis of quantitative metric characters. Size independent discriminant analysis compared a set of three morphological characters of the wing (length of veins delta and alpha and width of wing) measured from known specimens to detect differences between the two species. Morphometric discriminant analysis allowed differentiation of the females of both species with a high degree of accuracy (canonical correlation = 0.97; P << 0.01). The discriminant equations obtained may represent a useful and practical complementary taxonomic tool to distinguish accurately between previously unidentified female specimens of L. ceferinoi and L erwindonaldoi by measuring just three wing characters; these data call even be analyzed in the field for epidemiological in situ Studies, aided by the widespread availability of laptops and statistical software.