Kinetics and thermodynamics of DNA polymerases with exonuclease proofreading

被引：17
|
作者：
Gaspard, Pierre [1 ]
机构：
[1] Univ Libre Bruxelles, Ctr Nonlinear Phenomena & Complex Syst, Code Postal 231,Campus Pl, B-1050 Brussels, Belgium
来源：
PHYSICAL REVIEW E | 2016年 / 93卷 / 04期
关键词：
NATURAL SELF-ORGANIZATION; DEOXYRIBONUCLEIC-ACID; ENZYMATIC-SYNTHESIS; NUCLEOTIDE INCORPORATION; REPLICATION FIDELITY; ESCHERICHIA-COLI; HYPERCYCLE; PRINCIPLE; POLYMERIZATION; MECHANISM;
D O I：
10.1103/PhysRevE.93.042420
中图分类号：
O35 [流体力学]; O53 [等离子体物理学];
学科分类号：
070204 ; 080103 ; 080704 ;
摘要：
Kinetic theory and thermodynamics are applied to DNA polymerases with exonuclease activity, taking into account the dependence of the rates on the previously incorporated nucleotide. The replication fidelity is shown to increase significantly thanks to this dependence at the basis of the mechanism of exonuclease proofreading. In particular, this dependence can provide up to a 100-fold lowering of the error probability under physiological conditions. Theory is compared with numerical simulations for the DNA polymerases of T7 viruses and human mitochondria.
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页数：13
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