Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging

被引：2

作者：

Bub, Gil ^{[1
]}

Tecza, Matthias ^{[2
]}

Helmes, Michiel ^{[1
]}

Lee, Peter ^{[1
]}

Kohl, Peter ^{[1
]}

机构：

[1] Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 2JD, England

[2] Univ Oxford, Dept Phys, Oxford OX1 2JD, England

来源：

NATURE METHODS | 2010年 / 7卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

SIGNALS; RAT;

D O I：

10.1038/NMETH.1429

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.

引用

页码：209 / U66

页数：5

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