Inhibition of HIV-1 Infection by CCR5Delta32 Protein Expressed in Human PBMCs

被引：2
|
作者：
An Qun-Xing [1 ]
Lei Ying-Feng [2 ]
Yang Jing [2 ]
Zhang Xian-Qing [1 ]
Yi Jing [1 ]
Chen Rui [1 ]
Mu Shi-Jie [1 ]
机构：
[1] Fourth Mil Med Univ, Dept Blood Transfus, Xijing Hosp, Xian 710033, Peoples R China
[2] Fourth Mil Med Univ, Dept Microbiol, Xian 710032, Peoples R China
来源：
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2009年 / 36卷 / 09期
基金：
中国国家自然科学基金;
关键词：
CCR5Delta32; peripheral blood mononuclear cells; human immunodeficiency virus type 1; coreceptor; RESISTANCE; INDIVIDUALS; ALLELE;
D O I：
10.3724/SP.J.1206.2009.00083
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Individuals with CCR5Delta32 mutant genotype can naturally resist human immunodeficiency virus type 1 (HIV-1) infection. The mechanism is mainly due to CCR5Delta32 mutant protein expressed in the individual peripheral blood mononuclear cells (PBMCs), through trans-dominant negative (TDN) effect, which can inhibit two types of HIV-1 coreceptor (CCR5 and CXCR4) on the cell surface from producing. The recombinant lentiviruses, namely Lenti-CMDelta32, were generated and used to infect human PBMCs. HIV-1 infection of the PBMCs transduced with Lenti-CMDelta32 showed that CCR5Delta32 protein expressed in human PBMCs was able to inhibit R5, X4, and R5X4 HIV-1 infection. The result is expected to be used for the gene therapy on AIDS, which deserves further study.
引用
收藏
页码：1141 / 1145
页数：5
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