HPLC separation and determination of enantiomeric purity of novel nucleoside analogs, on cyclodextrin chiral stationary phases, using reversed and polar organic modes

This work reports a method for the determination of the purity of some potential antiviral agents by analytical HPLC. Such agents are nucleoside analogs, which possess one chiral center and are synthesized by an asymmetric route to give a single enantiomer (either R or S). Chiral stationary phases chosen for this study were Cyclobond 12000 [beta-cyclodextrin (beta-CD)] and Cyclobond 1 2000 RSP (R,S hydroxypropylether beta-CD). Resolutions were made using a reverse-phase methodology and polar organic mode. Furthermore, the effects of structural features were thoroughly studied in relation to retention, selectivity, resolution, and order of elution.