Simultaneous determination of mycophenolic acid and valproic acid based on derivatization by high-performance liquid chromatography with fluorescence detection

被引:28
|
作者
Zhong, Y
Jiao, Z
Yu, YQ
机构
[1] Fudan Univ, Sch Pharm, Shanghai 200032, Peoples R China
[2] Fudan Univ, Huashan Hosp, Dept Pharm, Shanghai 20040, Peoples R China
关键词
mycophenolic acid; valproic acid; derivatization; solvatochromism; fluorescence detection;
D O I
10.1002/bmc.566
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A reliable and validated reversed-phase high-performance liquid chromatography (HPLC) method using fluorescence detection is reported for the simultaneous quantitation of mycophenolic acid (MPA) and valproic acid (VPA) in human plasma. The method is based on the pre-column derivatization of valproic acid with 4-bromomethy-6, 7-dimethoxycoumarin (BrMMC) and online solvatochromism, of MPA by pH adjustment. The linear calibration range was 0.50-30 mu g/mL for MPA and 5.00-150 mu g/mL for VPA. The relative standard deviations of the method of intra- and inter-day analyses (n = 6) were below 6.5 and 6.7% for MPA, and 5.8 and 6.3% for VPA, respectively. Dichloromethane was used for the simultaneous extraction of MPA and VPA from acidified plasma. This reliable method can be applied in the analysis of MPA and VPA in human plasma using only a small volume (100 mu L). Copyright (c) 2005 John Wiley & Sons, Ltd.
引用
收藏
页码:319 / 326
页数:8
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