Sulfate-sulfur was applied to sulfur (S)-deficient canola at several growth stages in a field experiment at Cargo near Orange, New South Wales. Applications of 0, 10 or 40 kg S/ha (S-0, S-10 and S-40) as mixtures of potassium sulfate and potassium chloride were made at sowing, the 5-6 leaf rosette stage, flower buds visible, stem elongation and first flowering. The plots received either 80 or 160 kg nitrogen (N)/ha at sowing. Plants from the S-0 plots showed symptoms of severe S deficiency during rapid stem elongation, and had a 52% reduction in seed yield and a 21% reduction in seed oil concentration compared with the S-40 plants. Application of S-10 at sowing, or topdressing S-deficient plants with this rate of S, was inadequate because, although seed oil concentrations were normal (39-42%), seed yields were 25% lower than those from plots that received S-40. Topdressing S-deficient plants with S-40 at either the 5-6 leaf rosette stage, flower buds visible or stem elongation resulted in the same seed yields and seed oil concentrations as obtained when S-40 Was applied at sowing. However, there was a 15% reduction in seed yield but no reduction in seed oil concentration when the S-40 topdressing was delayed until flowering. Although S-10 Was inadequate to correct the S deficiency, there was no reduction in either seed yield or seed oil concentration when S-10 was topdressed as late as flowering, when compared with this rate of S applied at sowing. Seed meal protein levels were increased by the S-40 topdressings. Concentrations of S in seed from the S-0 and S-10 plants were below the critical value of 0.36% for canola. Seed N:S concentration ratios of S-deficient plants were greater than 10, but 7.5 for plants which received adequate S. Total glucosinolates in seed were increased by the application of S, but the levels were still well below the limit set for the canola standard.