Proteomic Analysis of Plasma Membrane Vesicles

被引：41

作者：

Bauer, Brigitte ^{[1
]}

Davidson, Max ^{[2
]}

Orwar, Owe ^{[1
]}

机构：

[1] Chalmers, Dept Chem & Biol Engn, S-41296 Gothenburg, Sweden

[2] Nanoxis AB, S-41296 Gothenburg, Sweden

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 09期

基金：

瑞典研究理事会;

关键词：

membrane isolation; membrane proteins; plasma membranes; proteomics; vesicles; SECRETORY VESICLES; IMMUNOGLOBULIN-E; PROTEINS; VESICULATION; CELLS; RECEPTOR; LIPIDS; MICROPARTICLES; FRACTIONATION; INTEGRATION;

D O I：

10.1002/anie.200803898

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flow-cell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1656 / 1659

页数：4

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