Xylitol production from non-detoxified corncob hemicellulose acid hydrolysate by Candida tropicalis

被引:84
|
作者
Ping, Yuan [1 ]
Ling, Hong-Zhi [1 ]
Song, Gang [1 ]
Ge, Jing-Ping [1 ]
机构
[1] Heilongjiang Univ, Coll Life Sci, Key Lab Microbiol, Harbin 150080, Peoples R China
基金
中国国家自然科学基金;
关键词
Bioconversion; Corncob; Fermentation; Microbial growth; Substrate inhibition; Xylitol; BAGASSE HYDROLYSATE; XYLOSE REDUCTASE; DEHYDROGENASE; FERMENTATION; BEHAVIOR;
D O I
10.1016/j.bej.2013.03.022
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The interest on use of lignocellulose for producing chemicals is increasing as these feedstocks are low cost, renewable and widespread sources of sugars. Corncob is an attractive raw material for xylitol production due to its high content of xylan. In this study, hemicellulose hydrolysate from corncobs without detoxification was used for xylitol production by Candida tropicalis CCTCC M2012462. Compared with prepared xylose medium, xylitol production with dilute acid hydrolysate medium does not seem to influence specific xylose reductase activity. The decrease in xylitol productivity with dilute acid hydrolysate medium is a result of a lower biomass concentration and lag-phase time. It appears that biomass growth rate is essential for xylitol production. In xylitol fermentation with a low initial inhibitors concentration and substrate feeding strategy, a maximal xylitol concentration of 38.8 g l(-1) was obtained after 84 h of fermentation, giving a yield of 0.7 g g(-1) xylose and a productivity of 0.46 g l(-1) h(-1). (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:86 / 91
页数:6
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