MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway

被引：4
|
作者：
Yang, Wengang [1 ]
Zhao, Lei [1 ]
Xue, Song [1 ]
Liu, Jidong [1 ]
Shan, Jianggui [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Renji Hosp, Dept Cardiovasc Surg, Sch Med, Shanghai 200127, Peoples R China
来源：
ARCHIVES OF MEDICAL SCIENCE | 2022年 / 18卷 / 06期
关键词：
miRNA-21; Ajuba; Isl1; axis; differentiation; BMSCs; UP-REGULATION; ADIPOGENESIS; MICRORNAS;
D O I：
10.5114/aoms/154956
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Introduction: In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods: We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of miRNA-21, ajuba, Isl1 and cTnI was detected by RT-qPCR, WB and immunofluorescence staining in groups. Results: MiRNA-21 over-expression increased the expression of Isl1, and vice versa. Ajuba over-expression decreased the expression of Isl1, and vice versa. Ajuba negatively regulated the differentiation of BMSCs into cardiomyocyte-like cells. Conclusions: MiRNA-21 could regulate differentiation of bone marrow mesenchymal stem cells (BMSCs) to cardiomyocyte-like cells through the ajuba/Isl1 axis pathway.
引用
收藏
页码：1672 / 1677
页数：6
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