Combining multiphoton and CARS microscopy for skin imaging

被引：0

作者：

Breunig, H. G. ^{[1
,2
,3
]}

Weinigel, M. ^{[1
,2
]}

Kellner-Hoefer, M. ^{[1
,2
]}

Bueckle, R. ^{[1
,2
]}

Darvin, M. E. ^{[4
]}

Lademann, J. ^{[4
]}

Koenig, K. ^{[1
,2
,3
]}

机构：

[1] JenLab GmbH, Schillerstr 1, D-07745 Jena, Germany

[2] Sci Pk 2, Campus D 12, D-66123 Saarbrucken, Germany

[3] Univ Saarland, Fac Mechatron & Phys, Dept Biophoton & Laser Technol, D-66123 Saarbrucken, Germany

[4] Charite, Ctr Expt & Cutaneous Physiol CCP, Dept Dermatol & Allergol, D-10117 Berlin, Germany

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIII | 2013年 / 8588卷

关键词：

CARS; CARS imaging; non-linear microscopy; skin imaging; multiphoton imaging; lipids;

D O I：

10.1117/12.2001985

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Microscopic imaging based on multiphoton fluorescence, second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) imaging has been realized in one common platform which is appropriate for use in hospitals. The different optical modalities non-invasively provide in vivo images from human skin with subcellular resolution, at different depths based on endogenous fluorescent, SHG-active molecules as well as non-fluorescent molecules with vibrational resonances at 2845 cm(-1), in particular lipids. An overview of the system employing a Ti: sapphire laser and photonic crystal fiber to generate the excitation light as well as several imaging examples are presented.

引用

页数：7

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