Copy number variations (CNVs) in human pluripotent cell-derived neuroprogenitors

被引：7
|
作者：
Lopez Corrales, Nestor L.
Mrasek, Kristin [1 ]
Voigt, Martin [1 ]
Liehr, Thomas [1 ]
Kosyakova, Nadezda [1 ]
机构：
[1] Univ Jena, Jena Univ Hosp, Inst Human Genet, D-07743 Jena, Germany
来源：
GENE | 2012年 / 506卷 / 02期
关键词：
Copy number variations (CNVs); Induced pluripotent stem cells (iPSC); Neuroprogenitor cell lines (NPC); Array comparative genomic hybridization (aCGH); EMBRYONIC STEM-CELLS; COMPARATIVE GENOMIC HYBRIDIZATION; CHROMOSOMAL-ABNORMALITIES; SOMATIC MOSAICISM; HUMAN ESCS; INSTABILITY; CULTURE; REVEALS; LINES; AMPLIFICATION;
D O I：
10.1016/j.gene.2012.07.005
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Results from the analysis of copy number variations (CNVs) in human pluripotent cell-derived neuroprogenitor cell lines (hiPSC and hESC-derived NPC) are presented. Two different types of CNVs were detected: a) CNVs inherited from the original source of pluripotent cells (hESC and hiPSC) and b) CNVs detected either in the original source of pluripotent cells or in the derived NPC cell lines but not in both at the same time. Our data suggest that submicroscopic chromosomal changes happened during culture and manipulation of cells and those differentiation procedures could result in gains and losses of genomic regions in pluripotent cell-derived neuroprogenitors. Overall, the results indicate that even chromosomally stable stem cell lines would need to be analyzed in detail by high resolution methodologies before their clinical use. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：377 / 379
页数：3
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