Coinfection with Fusobacterium nucleatum can enhance the attachment and invasion of Porphyromonas gingivalis or Aggregatibacter actinomycetemcomitans to human gingival epithelial cells

被引:44
|
作者
Li, Yan [1 ,2 ]
Guo, Hongmei [1 ,2 ]
Wang, Xijun [1 ,2 ]
Lu, Yang [3 ]
Yang, Chunyu [4 ]
Yang, Pishan [1 ,2 ]
机构
[1] Shandong Univ, Sch Stomatol, Jinan 250012, Shandong, Peoples R China
[2] Shandong Prov Key Lab Oral Tissue Regenerat, Jinan, Peoples R China
[3] Jining Med Univ, Jining, Shandong, Peoples R China
[4] Weifang Med Univ, Weifang, Shandong, Peoples R China
关键词
Periodontitis; Porphyromonas gingivalis; Fusobacterium nucleatum; Aggregatibacter actinomycetemcomitans; Interleukin-8; Human beta-defensin-2; ORAL BACTERIA; COAGGREGATION; HEALTH; MODEL;
D O I
10.1016/j.archoralbio.2015.06.017
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective: This study was conducted to investigate effects of coinfection of Porphyromonas gingivalis (P. gingivalis) or Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) with Fusobacterium nucleatum (F. nucleatum) on their adhering and invasive capacity to human gingival epithelial cells as well as the expression of interleukin-8 (IL-8) and human beta-defensin-2 (hBD-2) in human gingival epithelial cells. Design: P. gingivalis and A. actinomycetemcomitans were tested for their ability to attach and invade a human gingival epithelial cell line (Ca9-22) alone or coinfecting with F. nucleatum. Also, expression levels of IL-8 and hBD-2 were detected respectively using enzyme linked immunosorbent assay (ELISA) and real-time reverse transcription PCR (RT-PCR) when Ca9-22 cells were infected with P. gingivalis and A. actinomycetemcomitans alone or coinfecting with F. nucleatum. Results: F. nucleatum, P. gingivalis and A. actinomycetemcomitans were allowed to adhere and invade Ca9-22 cells, either each strain alone or under coinfection. The adhering and invasive abilities of P. gingivalis and A. actinomycetemcomitans were significantly greater when they were coincubated with F. nucleatum (P < 0.05) than either of them alone. These enhancements were inhibited by galactose. In addition, P. gingivalis and A. actinomycetemcomitans inhibited the activation of IL-8 and hBD-2 by F. nucleatum. Also, galactose disrupted this inhibition on the expression of IL-8 and hBD-2. Conclusion: These results suggested coinfection with F. nucleatum can enhance adhesion and invasion of P. gingivalis and A. actinomyceterncomitans to Ca9-22 cells, as well as inhibition on host innate immune response. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1387 / 1393
页数:7
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